recombinant human argonaute 2 eif2c2 protein  (Sino Biological)

Journal: OncoTargets and therapy

Article Title: LncRNA ZNF667-AS1 Promotes ABLIM1 Expression by Adsorbing micro RNA-1290 to Suppress Nasopharyngeal Carcinoma Cell Progression

doi: 10.2147/OTT.S245554

Figure Lengend Snippet: ZNF667-AS1 competitively binds to miR-1290 . ( A ) Comparison of miRNA results with the possible binding of ZNF667-AS1 predicted by RNA22 and miRNAs up-regulated in GSE70970 microarray dataset, there are 2 miRNAs in the intersection. ( B ) The expression of miR-1290 in GSE70970 microarray dataset. ( C ) RNA22 predicts the binding site between ZNF667-AS1 and miR-1290 . ( D ) RT-qPCR utilized to detect the expression of miR-1290 in tumor and adjacent tissues (n = 36), * p < 0.05 vs adjacent tissues. ( E ) Dual-luciferase reporter gene assay to verify the binding relationship between ZNF667-AS1 and miR-1290 . ( F ) FISH for the detection of subcellular localization of ZNF667-AS1 in CNE-1 cells (×200). ( G ) RNA pull-down assay to verify the binding of ZNF667-AS1 with miR-1290 , * p < 0.05 vs the Bio-NC group. ( H ) RIP assay for the detection of whether ZNF667-AS1 interacted directly with AGO2 protein in CNE-1 cells, * p < 0.05 vs the IgG group; # p < 0.05 vs the MUT- miR-1290 group. Data are expressed as mean ± standard deviation. Paired t test is used for comparison of two groups. One-way analysis of variance is used for comparison among multiple groups. Each experiment was run in triplicate. Abbreviations: ZNF667-AS1 , zinc finger protein 667-antisense RNA 1; miR-1290 , microRNA-1290 ; RT-qPCR, reverse transcription quantitative polymerase chain reaction; FISH, fluorescence in situ hybridization; NC, negative control; RIP, RNA immunoprecipitation; AGO2, argonaute 2; IgG, immunoglobulin G; WT, wild type; MUT, mutant; DAPI, 4ʹ,6-diamidino-2-phenylindole.

Article Snippet: LncRNA ZNF667-AS1 bound to AGO2 protein (rabbit anti-AGO2, ab32381, 1:50) and IgG (ab109489, 1:100, both Abcam) was determined with a RIP kit (Millipore, Billerica, MA, USA).

Techniques: Comparison, Binding Assay, Microarray, Expressing, Quantitative RT-PCR, Luciferase, Reporter Gene Assay, Pull Down Assay, Standard Deviation, Reverse Transcription, Real-time Polymerase Chain Reaction, Fluorescence, In Situ Hybridization, Negative Control, RNA Immunoprecipitation, Mutagenesis

Journal: Journal of Nanobiotechnology

Article Title: Treatment of breast cancer with autophagy inhibitory microRNAs carried by AGO2-conjugated nanoparticles

doi: 10.1186/s12951-020-00615-4

Figure Lengend Snippet: Physical characterization and composition of SPION nanoparticles

Article Snippet: Human AGO2 (His-tag) (EIF2C2) recombinant protein was purchased from Sino Biological Inc. (China).

Techniques:

Journal: Methods in molecular biology (Clifton, N.J.)

Article Title: A Novel Method to Silence Genes in Cryptosporidium

doi: 10.1007/978-1-4939-9748-0_11

Figure Lengend Snippet: Silencing genes in Cryptosporidium. (a) Assembling: hAgo2 protein is loaded with Cryptosporidium ssRNA complementary to mRNA target. (b) Encapsulation: hAgo2–ssRNA complex is encapsulated within liposomes (protein transfection reagent). (c) Transfection: Cryptosporidium oocysts are transfected with complexes. (d) Silencing: hAgo2–ssRNA binds to mRNA target, translation is blocked, and mRNA target is sliced by hAgo2, then expression of target is reduced

Article Snippet: Recombinant protein human Argonaute 2 (hAgo2) from human (Sino Biologicals, North Wales, PA).

Techniques: Transfection, Expressing